Composite

Part:BBa_K2027013:Design

Designed by: Michael Becich   Group: iGEM16_Stanford-Brown   (2016-10-08)


Mutant Feedback-Resistant Version of AroG (Pro150Leu)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241


Design Notes

Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant

Sequence Verification

Source

https://parts.igem.org/Part:BBa_K1060000

References